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With line selections, the following parameters can be recorded: length, angle (straight lines only), mean, Use the Analyze>Set Measurements command to specify what area statistics are recorded. Measured to calculate the absolute and relative tissue areas you wish

Use basic arithmatic relationships between the areas you have Make sure you save your results excel sheet under a unique name for further processing! Note to collect an image like the one above, you have to use SnagIt to capture the displayed window. One excel results sheet and display outlines of all areas measured in "Display Results" and "Add to Manager" to collect all measurements in Image>Adjust>Theshold (0-125 seems to work well on masks created above) to theshold the tissue/area to be measuredĪnalyze>Set Measurements (Select measurements you want to collect)Īnalyze>Analyze Particles (Set Analyze Radio Buttons: Make sure you save this Mask with a unique name! To incorporate, document what Tissue you measured in your exercise) Use Polygon Tool to Outline Entire Organ or Individual Tissues, that you want to measure.Įdit>Selection>Fit Spline (to smooth things out)Įdit>Selection>Create Mask ( generate an outline, image of this ImageJ will have automatically filled in the Distance in Pixelsįield based on the length of the line selection. The known distance and unit of measurement, then click OK. Then, bring up the Set Scale dialog, enter Line selection tool to make a line selection that corresponds toĢ. Before using this command, use the straight Measurement results can be presented in calibrated units,ġ. Use this dialog to define the spatial scale of the active image so Relative tissue areas you wish to present in your final lab exercises. Make sure you save your results excel sheet under a unique name for further processing!īetween the areas you have measured to calculate the absolute and Measurements in one excel results sheet and display outlines of all "Display Results" and "Add to Manager" to collect all Analyze>Analyze Particles (Set Analyze Radio Buttons: Analyze>Set Measurements (Select measurements you want to collect)